WORKSHOP ON

“BASIC NEUROCHEMICAL TECHNIQUES FOR YOUNG NEUROSCIENTISTS”

A maximum of 20 participants will be selected for the workshop. Students and junior faculty working in the area of Neuroscience are encouraged to apply. The selected participants will receive partial travel allowance within india and free local hospitality. The faculty of this workshop will comprise several national as well as international researchers in this field.

Venue: Department of Biotechnology, GNDU Amritsar

Convener:

Prof. Gurcharan Kaur,

Organizing Secretary,

Professor and Head,

Department of Biotechnology, Guru Nanak Dev University,

Amritsar-143005, INDIA.

Phone: 0183-2258431; Fax: 0183-2258272; Email: kgurcharan.neuro@yahoo.com

Conference email: snciamritsar@gmail.com

Proposed hands-on techniques for the workshop:

1. Mitochondrial isolation, electrophoresis and western blotting for molecular chaperon protein Mortalin expression in brain tissue.

2. Immunohistofluorescent localization for molecular marker of brain plasticity, Neural Cell Adhesion Molecule (NCAM) and Glial Fibrillary Acidic Protein (GFAP) expression in the discrete regions of the rat brain.

3. Establishing primary neuronal-glial cultures from rat cortex.

4. Fluorescent In Situ Hybridisation (FISH) for mRNA expression studies of plasticity marker proteins.

5. Extraction of total RNA from brain tissue and Northern Blotting.

6. Determination of specific RNA transcripts using RT-PCR.